6LIR
crystal structure of chicken TCR for 2.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-01 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.430, 83.097, 82.416 |
| Unit cell angles | 90.00, 117.72, 90.00 |
Refinement procedure
| Resolution | 38.744 - 2.091 |
| Rwork | 0.205 |
| R-free | 0.23590 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.091 | 2.091 |
| Rmerge | 0.114 | 0.860 |
| Number of reflections | 54200 | 52151 |
| <I/σ(I)> | 15.1 | 3.263 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 7.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.25M Lithium citrate tribasic tetrahydrate 24%PEG3350 |
