6LIK

Crystal Structure of Lectin from Pleurotus ostreatus in complex with Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-01
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.99
Spacegroup name	P 61 2 2
Unit cell lengths	152.507, 152.507, 146.062
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.040 - 2.400
R-factor	0.1862
Rwork	0.185
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kbq
RMSD bond length	0.014
RMSD bond angle	2.041
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.040	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.146
Rpim	0.043	0.423
Number of reflections	39208	3803
<I/σ(I)>	12.98	1.58
Completeness [%]	99.0
Redundancy	5.1
CC(1/2)	0.994	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	300	0.9M Na2HPO4/K2HPO4, pH 5.5, 33% glycerol