6LIK
Crystal Structure of Lectin from Pleurotus ostreatus in complex with Galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 152.507, 152.507, 146.062 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.040 - 2.400 |
| R-factor | 0.1862 |
| Rwork | 0.185 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kbq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.041 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.146 | |
| Rpim | 0.043 | 0.423 |
| Number of reflections | 39208 | 3803 |
| <I/σ(I)> | 12.98 | 1.58 |
| Completeness [%] | 99.0 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.994 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 300 | 0.9M Na2HPO4/K2HPO4, pH 5.5, 33% glycerol |
