6LHZ
Structure of aerolysin-like protein (Bombina maxima)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 79.082, 79.082, 48.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.720 - 2.520 |
R-factor | 0.1969 |
Rwork | 0.193 |
R-free | 0.26450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lh8 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.117 | 0.492 |
Number of reflections | 5562 | 3919 |
<I/σ(I)> | 18.5 | 12.571 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.6 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 289 | 0.2 M magnesium chloride hexahydrate, 25% polyethylene glycol 3350, 0.1 M Bis-tris |