6LHF
Crystal structure of chicken cCD8aa/pBF2*15:01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97972 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.340, 66.735, 103.949 |
| Unit cell angles | 84.59, 82.04, 67.51 |
Refinement procedure
| Resolution | 29.893 - 2.700 |
| Rwork | 0.253 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e0r |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.109 | 0.516 |
| Number of reflections | 36586 | 108500 |
| <I/σ(I)> | 11.587 | |
| Completeness [%] | 95.8 | 98 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium chloride, 25% (w/v) polyethylene glycol 3,350,0.1 M HEPES pH 7.5 |
