6LH8

Structure of aerolysin-like protein (Bombina maxima)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 4 21 2
Unit cell lengths	78.793, 78.793, 48.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.578 - 1.729
R-factor	0.196
Rwork	0.192
R-free	0.23110
Structure solution method	SAD
Data scaling software	SCALEPACK (HKL2000)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.580	1.760
High resolution limit [Å]	1.729	1.729
Number of reflections	16266	1522
<I/σ(I)>	41.88	2.62
Completeness [%]	100.0	100
Redundancy	13.3
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	289	0.2M magnesium chloride hexahydrate, 25% PEG 3350, 0.1M bis-tris