6LGD
Bombyx mori GH13 sucrose hydrolase complexed with 1,4-dideoxy-1,4-imino-D-arabinitol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.054, 146.444, 153.291 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.290 - 1.750 |
| R-factor | 0.1632 |
| Rwork | 0.162 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5brq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.074 | 0.889 |
| Number of reflections | 148203 | 21397 |
| <I/σ(I)> | 15.4 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.7 |
| CC(1/2) | 0.999 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% PEG 3350, 0.2 M magnesium acetate, 10 mM 1,4-dideoxy-1,4-imino-D-arabinitol |
