6LFV
Crystal structure of PCB4scFv(hN56D)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 92.420, 92.420, 53.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.780 - 1.900 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3esu |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.521 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.780 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.527 |
| Number of reflections | 41553 | 1322 |
| <I/σ(I)> | 25.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, PEG 8000, ethylene glycol |
