6LFU

Poa1p F152A mutant in complex with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-02
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	46.399, 68.509, 55.879
Unit cell angles	90.00, 91.68, 90.00

Refinement procedure

Resolution	24.884 - 3.123
R-factor	0.1954
Rwork	0.188
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lfq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.884	3.436
High resolution limit [Å]	3.123	3.123
Number of reflections	5850
<I/σ(I)>	20.5
Completeness [%]	98.0
Redundancy	3.5
CC(1/2)	0.985	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	Citric acid, PEG 3350, Glycerol