6LFR
Poa1p in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-26 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 64 |
| Unit cell lengths | 85.619, 85.619, 37.945 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.518 - 1.780 |
| R-factor | 0.1781 |
| Rwork | 0.175 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lfq |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.518 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 15302 | |
| <I/σ(I)> | 51.3 | |
| Completeness [%] | 99.2 | |
| Redundancy | 9.7 | |
| CC(1/2) | 0.985 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | Ammonium sulfate, Sodium cacodylate trihydrate, PEG 8000, Glycerol |
