6LF9
Crystal structure of pSLA-1*1301 complex with dodecapeptide RVEDVTNTAEYW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.169, 44.240, 199.631 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 199.630 - 2.500 |
| R-factor | 0.25291 |
| Rwork | 0.251 |
| R-free | 0.29127 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 199.630 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.176 | 0.426 |
| Number of reflections | 55584 | 43280 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2 M Ammonium fluoride, 20% w/v Polyethylene glycol 3,350 |
