6LF1
SeviL, a GM1b/asialo-GM1 binding lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 86.216, 86.216, 67.627 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.110 - 1.700 |
| R-factor | 0.1942 |
| Rwork | 0.193 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i40 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.736 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.110 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.038 | 0.385 |
| Number of reflections | 371509 | 1641 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 98.3 | 97.1 |
| Redundancy | 11.7 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.1 M citric acid, pH 3.5, 3 M NaCl |
