6LF0

Structure of FEM1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-01-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	88.720, 98.480, 146.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.502 - 2.110
R-factor	0.1966
Rwork	0.195
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lbf
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.740	2.160
High resolution limit [Å]	2.110	2.110
Number of reflections	74445	7352
<I/σ(I)>	19.7
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Lithium sulfate, 0.1M Tris-HCl pH 7.3