6LEN
Structure of NS11 bound FEM1C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-07-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.727, 96.508, 146.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.652 - 2.383 |
R-factor | 0.2006 |
Rwork | 0.199 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lbf |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.652 | 2.510 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 54996 | 5405 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 100.0 | |
Redundancy | 13.1 | |
CC(1/2) | 0.998 | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Sodium citrate tribasic dihydrate, 0.1M Tris pH 8.2 |