6LEA
Structure of FliS chaperone in complex with flagellin and HP1076
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 61 |
Unit cell lengths | 103.330, 103.330, 144.211 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.620 - 2.950 |
R-factor | 0.1954 |
Rwork | 0.190 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k1i |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17rc1_3605) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.620 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.091 | 0.452 |
Number of reflections | 16741 | 2628 |
<I/σ(I)> | 16.6 | |
Completeness [%] | 90.9 | |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 3% (v/v) PEG 20000, 0.2M Potassium bromide, 0.2M Potassium thiocyanate, 0.2M Sodium acetate pH 5.0 |