6LDY
Structure antibody D6 in complex with methylated peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.520, 76.210, 79.210 |
| Unit cell angles | 85.58, 84.20, 76.03 |
Refinement procedure
| Resolution | 40.180 - 1.770 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ldv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.727 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.180 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.087 | 0.517 |
| Rpim | 0.056 | 0.335 |
| Number of reflections | 88438 | 12726 |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 96.9 | 95.3 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.993 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Calcium chloride 20% PEG3350 |
