6LDY

Structure antibody D6 in complex with methylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	41.520, 76.210, 79.210
Unit cell angles	85.58, 84.20, 76.03

Refinement procedure

Resolution	40.180 - 1.770
R-factor	0.1864
Rwork	0.185
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ldv
RMSD bond length	0.012
RMSD bond angle	1.727
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.180	1.870
High resolution limit [Å]	1.770	1.770
Rmerge	0.087	0.517
Rpim	0.056	0.335
Number of reflections	88438	12726
<I/σ(I)>	8.4	2.1
Completeness [%]	96.9	95.3
Redundancy	3.3	3.3
CC(1/2)	0.993	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M Calcium chloride 20% PEG3350