6LDX

Structure antibody E6 in complex with methylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-16
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	82.329, 82.329, 120.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.940 - 1.800
R-factor	0.1713
Rwork	0.169
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ldv
RMSD bond length	0.014
RMSD bond angle	1.857
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.940	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.092	0.468
Rpim	0.019	0.131
Number of reflections	39032	5534
<I/σ(I)>	23.3	5.6
Completeness [%]	99.9	99.1
Redundancy	23.2	13.5
CC(1/2)	0.999	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M potassium chloride 24% PEG3350