6LDN

Crystal structure of T.onnurineus Csm5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	95
Detector technology	CCD
Collection date	2017-09-21
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	61.473, 131.072, 51.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.770 - 2.600
R-factor	0.2134
Rwork	0.208
R-free	0.26330
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	0.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.770	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	13324	13324
<I/σ(I)>	61.49
Completeness [%]	99.6
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	20 % PEG 8000 (w/v), 100 mM CHES/sodium hydroxide pH 9.5