6LCT

Crystal structure of catalytic inactive chloroplast resolvase NtMOC1 in complex with Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	121.054, 89.807, 68.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.350 - 2.550
R-factor	0.2211
Rwork	0.220
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kvo
RMSD bond length	0.003
RMSD bond angle	0.517
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.350	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.030	0.727
Rmeas	0.036	0.789
Rpim	0.014	0.418
Number of reflections	24991	2962
<I/σ(I)>	28.2	2.6
Completeness [%]	99.9
Redundancy	6.5	6.7
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	PEG 4000, Sodium acetate trihydrate, 3-Methyl-1,5-Pentane Diol, D-sorbitol