6LCM

Crystal structure of chloroplast resolvase ZmMOC1 with the magic triangle I3C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-02
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	49.136, 49.136, 141.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.500
R-factor	0.2256
Rwork	0.223
R-free	0.27990
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.684
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXDE
Refinement software	PHENIX (v1.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.092	0.302
Number of reflections	6571	670
<I/σ(I)>	70.8
Completeness [%]	100.0
Redundancy	33.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Ammonium fluoride