6LCG
Structure of D-carbamoylase mutant from Nitratireductor indicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 18-ID |
| Synchrotron site | APS |
| Beamline | 18-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2019-11-11 |
| Detector | Bruker AXIOM 200 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 95.650, 95.650, 261.621 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.820 - 2.700 |
| R-factor | 0.2184 |
| Rwork | 0.216 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fo6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.426 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.229 | 2.257 |
| Number of reflections | 20291 | 2616 |
| <I/σ(I)> | 1.51 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | PEG 4000, Sodium Chlorium, Tris-HCl |
