6LCE

Crystal Structure of beta-L-arabinobiose binding protein - selenomethionine derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	53.339, 66.277, 92.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.200 - 1.780
R-factor	0.1415
Rwork	0.139
R-free	0.18220
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.749
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	1.820
High resolution limit [Å]	1.780	1.780
Rmerge	0.161	0.947
Number of reflections	31327	1476
<I/σ(I)>	18.3	2.3
Completeness [%]	98.0
Redundancy	17.4
CC(1/2)	0.998	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293	18% PEG 1000, 0.1M Na-citrate (pH 3.5), 0.6mM beta-L-arabinobiose