6LBU

Crystal structure of yeast Stn1 and Ten1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	45.306, 78.031, 101.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.951 - 2.000
R-factor	0.2135
Rwork	0.211
R-free	0.25750
Structure solution method	SAD
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.088	0.062	0.372
Rmeas	0.092	0.064	0.389
Rpim	0.026	0.018	0.110
Total number of observations	314501
Number of reflections	24869	2698	2338
<I/σ(I)>	4.6
Completeness [%]	99.5	100	95.7
Redundancy	12.6	12.1	11.3
CC(1/2)		0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	277	0.2 M potassium chloride, 0.05 M Sodium cacodylate trihydrate, 10% (w/v) PEG4000