6LA8

349 bp di-nucleosome harboring cohesive DNA termini assembled with linker histone H1.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2018-09-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	121.055, 184.073, 223.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.320 - 3.400
R-factor	0.2076
Rwork	0.206
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lab 4qlc
RMSD bond length	0.006
RMSD bond angle	1.262
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.320	3.590
High resolution limit [Å]	3.400	3.400
Rmerge	0.151
Rmeas	0.163
Rpim	0.062
Number of reflections	69141	9843
<I/σ(I)>	12.1	1.1
Completeness [%]	99.8
Redundancy	13.3
CC(1/2)	1.000	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291.15	Calcium chloride, potassium chloride, sodium acetate (cryo-25% MPD)