6L9Z

338 bp di-nucleosome assembled with linker histone H1.X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98
Detector technology	PIXEL
Collection date	2017-09-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	103.500, 101.410, 215.670
Unit cell angles	90.00, 97.48, 90.00

Refinement procedure

Resolution	213.830 - 2.500
R-factor	0.2048
Rwork	0.204
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ut9 2lso
RMSD bond length	0.009
RMSD bond angle	1.432
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	213.830	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.099
Rmeas	0.120
Rpim	0.066
Number of reflections	150292	20617
<I/σ(I)>	9.1
Completeness [%]	98.3
Redundancy	5.9
CC(1/2)	0.999	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	291	Calcium chloride, Potassium chloride, Potassium Cacodylate