6L98

Crystalline cast nephropathy-causing Bence-Jones protein AK: An entire immunoglobulin lambda light chain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	61.379, 71.794, 98.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.860 - 1.770
R-factor	0.2308
Rwork	0.229
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hk0
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.770	1.770
Number of reflections	42953	4233
<I/σ(I)>	18.8
Completeness [%]	99.9	100
Redundancy	6.5	6.7
CC(1/2)	0.596	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	100 mM MES 15% glycerol 15% PEG6000