6L97

Complex of DNA polymerase IV and L-DNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	85.861, 54.969, 96.545
Unit cell angles	90.00, 113.39, 90.00

Refinement procedure

Resolution	34.496 - 2.362
R-factor	0.232
Rwork	0.230
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bq3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.360	5.080	2.360
Rmerge	0.125	0.080	0.555
Rmeas	0.140	0.090	0.656
Rpim	0.062	0.041	0.342
Total number of observations	153932
Number of reflections	33537	3352	3238
<I/σ(I)>	18.4
Completeness [%]	97.9	94.6	95.3
Redundancy	4.6	5	3
CC(1/2)		0.989	0.164

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M Sodium formate, 0.05 M BICINE (pH 8.5), 10% w/v Polyethylene glycol monomethyl ether 5,000, and 1.1% benzamidine-HCl