6L8S

High resolution crystal structure of crustacean hemocyanin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	119.454, 207.596, 187.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.695 - 1.580
R-factor	0.1507
Rwork	0.149
R-free	0.18620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hcy
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.695	1.680
High resolution limit [Å]	1.580	1.580
Rmerge		0.464
Number of reflections	615236	99005
<I/σ(I)>	15.24
Completeness [%]	99.7
Redundancy	3.78
CC(1/2)	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Lithium sulfate monohydrate, Sodium citrate tribasic dihydrate, PEG1,000, magnesium chloride,