6L8P

Crystal structure of RidA from Antarctic bacterium Psychrobacter sp. PAMC 21119

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	67.284, 71.141, 108.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.227 - 1.601
R-factor	0.1487
Rwork	0.148
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k0t
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.106	0.069	0.486
Rmeas	0.115	0.075	0.523
Rpim	0.044	0.029	0.193
Total number of observations	479686
Number of reflections	69280	3727	3385
<I/σ(I)>	10.6
Completeness [%]	100.0	99.8	100
Redundancy	6.9	6.9	7.4
CC(1/2)		0.995	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris Propane/HCl (pH 7), 1.1 M malonic acid, 0.15 M ammonium citrate tribasic, 0.072 M succinic acid, 0.18 M DL-malic acid, 0.24 M sodium acetate, 0.3 M sodium formate, and 0.1 M ammonium tartrate dibasic