6L8K
Structure of URT1 in complex with UTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.766, 64.928, 66.371 |
Unit cell angles | 90.00, 121.19, 90.00 |
Refinement procedure
Resolution | 30.627 - 2.999 |
R-factor | 0.1927 |
Rwork | 0.190 |
R-free | 0.23960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w0n |
RMSD bond length | 0.002 |
RMSD bond angle | 0.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.627 | 3.400 |
High resolution limit [Å] | 2.999 | 2.999 |
Rmerge | 0.106 | 0.499 |
Number of reflections | 8925 | 2924 |
<I/σ(I)> | 8.857 | |
Completeness [%] | 98.6 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES PH7.5, 10% PEG6000, 5% MPD |