6L8E
Crystal structure of heterohexameric YoeB-YefM complex bound to 26bp-DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 151.352, 151.352, 183.778 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.510 - 2.350 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a6q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.658 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.480 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.078 | 1.070 |
Rmeas | 0.083 | 1.147 |
Rpim | 0.030 | 0.403 |
Number of reflections | 89409 | 4434 |
<I/σ(I)> | 17.4 | 2.5 |
Completeness [%] | 99.8 | |
Redundancy | 7.4 | |
CC(1/2) | 0.999 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 8000, 8% v/v Ethylene glycol |