6L8D

Hexameric structure of the ATPase subunit of magnesium chelatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	211.034, 121.149, 119.419
Unit cell angles	90.00, 99.06, 90.00

Refinement procedure

Resolution	49.330 - 2.910
R-factor	0.234
Rwork	0.233
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g8p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.076	0.731
Number of reflections	64295	6437
<I/σ(I)>	17.8	2
Completeness [%]	98.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	sodium malonate, PEG 3,350.