6L8B
The ligand-free structure of human PPARgamma LBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97941 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.150, 62.256, 119.504 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 29.345 - 2.102 |
| Rwork | 0.218 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vso |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.101 | 0.583 |
| Number of reflections | 38322 | 1932 |
| <I/σ(I)> | 12.625 | |
| Completeness [%] | 98.0 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 1.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES pH 7.5 |
