6L85

The sodium-dependent phosphate transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-03
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	121.178, 112.650, 110.792
Unit cell angles	90.00, 119.36, 90.00

Refinement procedure

Resolution	29.399 - 2.302
R-factor	0.1898
Rwork	0.188
R-free	0.22410
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.044	0.024	0.355
Rmeas	0.052	0.028	0.423
Rpim	0.026	0.014	0.227
Total number of observations	208830
Number of reflections	56236	5742	5193
<I/σ(I)>	12.4
Completeness [%]	98.1	98.2	91.2
Redundancy	3.7	3.7	3
CC(1/2)		0.999	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50mM sodium citrate (pH 5.5), 100mM NaCl, 18-20% (w/v) pentaerythritol propoxylate (5/4 PO/OH) and 10-12% polyethylene glycol 300