6L82

Crystal structure of Chaetomium GCP5 N-terminus and Mozart1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-16
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9998
Spacegroup name	C 2 2 21
Unit cell lengths	83.760, 101.878, 51.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.903 - 2.241
R-factor	0.21587415806
Rwork	0.211
R-free	0.26309
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l7r
RMSD bond length	0.010
RMSD bond angle	0.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.330
High resolution limit [Å]	2.200	2.250
Number of reflections	77073	10652
<I/σ(I)>	14.2
Completeness [%]	97.3
Redundancy	7.2
CC(1/2)	0.997	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1% tryptone, 0.05 M HEPES sodium pH 7.0, 0.001 M sodium azide, 20% PEG 3350