6L7H

crystal structure of GgCGT in complex with UDP and Nothofagin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54056
Spacegroup name	P 21 21 21
Unit cell lengths	54.193, 71.306, 110.022
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.002 - 1.800
R-factor	0.1717
Rwork	0.169
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l5p
Data reduction software	CrysalisPro
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.838	9.002	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge		0.057	0.621
Rmeas	0.107	0.061	0.703
Rpim	0.041	0.022	0.322
Total number of observations	256181	7794	26316
Number of reflections	39867	1029	5750
<I/σ(I)>	12.5	28.5	2.1
Completeness [%]	99.0	74.4	99.3
Redundancy	6.4	7.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	0.2 M Ammonium acetate, 0.1 M BIS-Tris, pH 6.5, 25% w/v Polyethylene glycol 3350