6L6Y
Crystal Structure of Pluripotency Reprogramming Factor Sox17 mutant (Sox17EK) HMG Domain bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-07-12 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.040, 73.205, 81.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.970 - 3.000 |
| R-factor | 0.1961 |
| Rwork | 0.190 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f27 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.256 |
| Data reduction software | PROTEUM |
| Data scaling software | SADABS |
| Phasing software | PHENIX (1.17_3644) |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.970 | 3.090 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.170 | 0.450 |
| Number of reflections | 16478 | 839 |
| <I/σ(I)> | 11.02 | 2.74 |
| Completeness [%] | 99.1 | 95.3 |
| Redundancy | 9.61 | 7.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 0.2 M Ammonium Sulfate, 30% PEG 4000, 0.1 M Tris pH 8.6 |
