6L6Q

Structural basis of NR4A2 homodimers binding to selective Nur-responsive elements

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 43
Unit cell lengths	35.696, 35.696, 259.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.409 - 2.601
R-factor	0.2553
Rwork	0.249
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cit
RMSD bond length	0.006
RMSD bond angle	0.866
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.410	2.693
High resolution limit [Å]	2.601	2.601
Rmerge	0.075	0.555
Rmeas	0.077
Number of reflections	9915	990
<I/σ(I)>	16.81	4.42
Completeness [%]	100.0
Redundancy	13.8
CC(1/2)	1.000	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	150 mM NaCl, 50 mM MES (pH 5.93), 10 mM MgCl2, 5 mM CaCl2, and 10-12% PEG4K (w/v)