6L6O
Crystal structure of stabilized Rab5a GTPase domain from Leishmania donovani
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-05-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.018, 58.018, 103.418 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.420 - 1.800 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hei |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.057 | 0.027 | 0.748 |
| Rmeas | 0.059 | 0.027 | 0.784 |
| Rpim | 0.015 | 0.007 | 0.223 |
| Total number of observations | 287720 | ||
| Number of reflections | 19056 | 2068 | 1701 |
| <I/σ(I)> | 16 | ||
| Completeness [%] | 98.9 | 99.5 | 89.7 |
| Redundancy | 15.1 | 17 | 8.8 |
| CC(1/2) | 1.000 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 200mM Ammonium Acetate, 100mM Tris HCl, pH 8.5 15% PEG 3350 |
