6L5S

crystal structure of GgCGT in complex with UDP-Glu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	53.376, 70.620, 108.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.484 - 1.914
R-factor	0.1604
Rwork	0.158
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2acw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.166	0.066	0.773
Rmeas	0.176	0.070	0.820
Rpim	0.056	0.022	0.269
Total number of observations	293646
Number of reflections	31904	1699	1545
<I/σ(I)>	3.9
Completeness [%]	99.5	96.6	100
Redundancy	9.2	8.9	8.8
CC(1/2)		0.998	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v Polyethylene glycol 3350