6L5R
crystal structure of GgCGT in complex with UDP-Glu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.750, 67.336, 105.756 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.668 - 2.890 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.26110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2acw |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.950 |
High resolution limit [Å] | 2.890 | 7.850 | 2.900 |
Rmerge | 0.197 | 0.068 | 0.848 |
Rmeas | 0.210 | 0.073 | 0.940 |
Rpim | 0.072 | 0.026 | 0.395 |
Number of reflections | 9044 | 513 | 419 |
<I/σ(I)> | 3.5 | ||
Completeness [%] | 99.7 | 100 | 96.5 |
Redundancy | 8.1 | 7.6 | 5.2 |
CC(1/2) | 0.996 | 0.427 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v Polyethylene glycol 3350 |