6L5O

Crystal structure of human DEAD-box RNA helicase DDX21 at post-hydrolysis state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97892
Spacegroup name	P 1 21 1
Unit cell lengths	66.460, 43.830, 72.440
Unit cell angles	90.00, 115.64, 90.00

Refinement procedure

Resolution	36.906 - 1.800
R-factor	0.1773
Rwork	0.175
R-free	0.20960
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.865
Data reduction software	HKL-2000
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.307	36.906	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge		0.046	0.673
Rmeas	0.145	0.052	0.765
Rpim	0.065	0.023	0.354
Total number of observations	139159	4453	19644
Number of reflections	33164	1037	4904
<I/σ(I)>	7.9	18.1	2.3
Completeness [%]	94.6	89	96.1
Redundancy	4.2	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% pEG3350, 0.2m Magnesium acetate