6L5M
Crystal structure of human DEAD-box RNA helicase DDX21 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.390, 219.070, 126.390 |
| Unit cell angles | 90.00, 90.79, 90.00 |
Refinement procedure
| Resolution | 36.510 - 2.700 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.378 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.682 | |
| Number of reflections | 51933 | 51933 |
| <I/σ(I)> | 6.4 | 0.9 |
| Completeness [%] | 95.8 | 99.7 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 19% pEG3350, 0.18M ammonium citrate, 40mM myo-inositol |
