6L5H

Crystal structure of human rootletin 1108-1200

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	139.088, 32.162, 36.997
Unit cell angles	90.00, 96.17, 90.00

Refinement procedure

Resolution	22.087 - 1.300
R-factor	0.1898
Rwork	0.189
R-free	0.20440
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.300	3.530	1.300
Rmerge	0.052	0.033	0.531
Rmeas	0.056	0.036	0.581
Rpim	0.021	0.014	0.231
Total number of observations	281721
Number of reflections	40235	2053	1999
<I/σ(I)>	14.6
Completeness [%]	99.8	97.2	98.4
Redundancy	7	6.8	5.9
CC(1/2)		0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1 M HEPES pH 7.0, 14% PEG 4000