6L3G

Structural Basis for DNA Unwinding at Forked dsDNA by two coordinating Pif1 helicases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 31 1 2
Unit cell lengths	131.265, 131.265, 117.183
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.566 - 3.300
R-factor	0.2472
Rwork	0.244
R-free	0.30310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fhd
Data reduction software	XFIT
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.700	3.560
High resolution limit [Å]	3.300	3.300
Rmerge	0.200
Number of reflections	17102	3547
<I/σ(I)>	7.1
Completeness [%]	97.9
Redundancy	6.2
CC(1/2)	0.982	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290	80 mM Sodium chloride, 40 mM Sodium cacodylate pH 6.0, 35% MPD, 12 mM Spermine