6L30

Crystal structure of the epithelial cell transforming 2 (ECT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	103
Detector technology	CCD
Collection date	2015-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	I 2 2 2
Unit cell lengths	106.959, 130.579, 131.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.851 - 2.800
R-factor	0.2384
Rwork	0.237
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n40 1lb1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.851	2.928
High resolution limit [Å]	2.800	2.800
Number of reflections	23040	2692
<I/σ(I)>	21.42
Completeness [%]	99.8
Redundancy	6.1
CC(1/2)	0.720	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	291	0.95-1.05 M Potassium/Sodium Tartrate 0.2 M Lithium Sulfate 1 mM TCEP