6L2Z

IlvC, a ketol-acid reductoisomerase, from Streptococcus pnuemoniae_D191G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-07
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	68.880, 104.511, 112.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.870 - 2.020
R-factor	0.2057
Rwork	0.204
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l2i
RMSD bond length	0.007
RMSD bond angle	0.878
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.870	2.090
High resolution limit [Å]	2.020	2.020
Number of reflections	52903	2650
<I/σ(I)>	54.6
Completeness [%]	98.1
Redundancy	13.2
CC(1/2)	0.996	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.1M HEPES pH 7.5, 0.1 M NaCl, 1.5 M ammonium sulfate, 15% glycerol