6L2W
Crystal structure of a novel fold protein Gp72 from the freshwater cyanophage Mic1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.702, 45.534, 67.368 |
| Unit cell angles | 90.00, 94.29, 90.00 |
Refinement procedure
| Resolution | 42.770 - 2.290 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.23850 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Rmerge | 0.083 | 0.301 |
| Number of reflections | 11607 | 1125 |
| <I/σ(I)> | 25.23 | 4.9 |
| Completeness [%] | 98.8 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 13% polyethylene glycol 6000, 0.1 M ADA pH 7.0, 0.01 M spermine tetrahydrochloride and 0.1 M guanidine hydrochloride |
