6L2M
The structure of the tRNA-specific deaminase mutant from M. capricolum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 55.589, 55.589, 109.227 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.141 - 2.303 |
R-factor | 0.201104412273 |
Rwork | 0.198 |
R-free | 0.25141 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l2l |
RMSD bond length | 0.007 |
RMSD bond angle | 0.813 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.105 | 0.510 |
Number of reflections | 9158 | 889 |
<I/σ(I)> | 38.5 | 4.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.7 | 18.1 |
CC(1/2) | 0.990 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 1.4M ammonium sulfate, 0.1M Sodium citrate pH 6.0 |