6L2L
The structure of the tRNA-specific deaminase from M. capricolum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-07 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.138, 49.138, 147.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.065 - 2.400 |
| R-factor | 0.190815182497 |
| Rwork | 0.189 |
| R-free | 0.22051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a8n |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.682 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.070 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.460 |
| Number of reflections | 7697 | 1081 |
| <I/σ(I)> | 15.6 | 4.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.6 | 7.1 |
| CC(1/2) | 0.998 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277 | 0.49M sodium phosphate monobasic monohydrate, 0.91M potassium phosphate dibasic, pH 6.9 |
