6L2G
Crystal structure of Aspergillus fumigatus mitochondrial acetyl-CoA acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.513, 174.780, 179.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.840 - 2.410 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f2s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.564 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 2.469 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.053 | 0.559 |
| Number of reflections | 73595 | 5143 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES pH 7.0, 2 M ammonium sulphate |
